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. 2021 Jul 7;108(8):1466–1477. doi: 10.1016/j.ajhg.2021.06.010

Figure 5.

Figure 5

DNAH2 and DNAH6 immunostaining is altered in spermatozoa obtained from men harboring bi-allelic DNAH10 variants

(A and B) The spermatozoa obtained from a fertile control individual (NC) and men harboring bi-allelic DNAH10 variants were stained with anti-DNAH2 (indicated as red in [A]), anti-DNAH6 (indicated as red in [B]), anti-a-tubulin (indicated as green) antibodies, and DAPI (indicated as blue). DNAH2 and DNAH6 normally localized along the sperm flagella in the control sperm. However, expression of both DNAH2 and DNAH6 was almost absent in the sperm obtained from men harboring bi-allelic DNAH10 variants. Scale bars, 10 μm.