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Primers design
| Genes | Primer sequences |
|---|---|
| GAPDH |
F: AACTTTGGTATCGTGGAAGG R: CAGTAGAGGCAGGGATGATGT |
| TNF-α |
F: CCTCACACTCACAAACCACCA R: ACAAGGTACAACCCATCGGC |
| IL1β |
F: CCACAGACCTTCCAGGAGAATG R: GTGCAGTTCAGTGATCGTACAGG |
| IL6 |
F: GCCTTCTTGGGACTGATGCT R: TGCCATTGCACAACTCTTTTCT |
| Occludin |
F: AACGATAACCTAGAGACACCT R: AATTATCACACATCAAGAGGA |
| Claudin-11 |
F: AAACCGTTTCTATTACTCTTC R: GACTGCGTCATGGCCACTGG |
| Connexin-43 |
F: GAACTCAAGGTTGCCCAAAC R: TTAGAGATGGTGCTTCCCG |
