Table 3.
Gradient setting for program 1
| Time (min) | Flowrate (mL/min) | A% | B% |
|---|---|---|---|
| Initial | 0.4 | 99 | 1 |
| 4 | 0.4 | 90 | 10 |
| 10 | 0.4 | 67 | 33 |
| 13 | 0.4 | 1 | 99 |
| 15 | 0.4 | 1 | 99 |
| 16.5 | 0.4 | 99 | 1 |
| 20 | 0.4 | 99 | 1 |
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