Table 5.
Gradient setting for program 2
| Time (min) | Flowrate (mL/min) | A% | B% |
|---|---|---|---|
| Initial | 0.4 | 80 | 20 |
| 2 | 0.4 | 80 | 20 |
| 3 | 0.4 | 20 | 80 |
| 5 | 0.4 | 20 | 80 |
| 6 | 0.4 | 80 | 20 |
| 10 | 0.4 | 80 | 20 |
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