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. 2021 Aug 12;2(3):100740. doi: 10.1016/j.xpro.2021.100740

Ascorbic acid and selenium stock (50X)

Reagent Final concentration Amount
L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate 50X 1.6 g
Sodium selenite stock (0.7 mg/mL) 50X 485 μL
DMEM/F12 N/A 499.515 mL
Total N/A 500 mL

Store aliquots at −80°C up to one year.