Table 1.
Sequences of primers designed for RT-qPCR.
| Gene | Source | Forward sequence | Reverse sequence |
|---|---|---|---|
| Nrf2 | Human | CATCCAGTCAGAAACCAGTGG | GCAGTCATCAAAGTACAAAGCAT |
| HO-1 | Human | GGGTGATAGAAGAGGCCAAGACT | CAGCTCCTGCAACTCCTCAAA |
| α-SMA | Human | TGTGGCTATCCAGGCGGTGC | TCTCGGCCAGCCAGATCCAGAC |
| COL1α1 | Human | GAGGGCCAAGACGAAGACATC | CAGATCACGTCATCGCACAAC |
| MMP3 | Human | TGCTTTGTCCTTTGATGCTG | GATTTTCCTCACGGTTGGAG |
| TIMP1 | Human | CTGTTGTTGCTGTGGCTGAT | GTTGTGGGACCTGTGGAAGT |
| GAPDH | Human | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA |
| Nrf2 | Mouse | AACAACGCCCTAAAGCA | TGGATTCACATAGGAGC |
| HO-1 | Mouse | CACGTATACCCGCTACCT | CCAGTTTCATTCGAGCA |
| α-SMA | Mouse | ATGAAGCCCAGAGCAAGAGA | TCCAGAGTCCAGCACAATACC |
| TGF-β1 | Mouse | TGACGTCACTGGAGTTGTACGG | GGTTCATGTCATGGATGGTGC |
| COL1α1 | Mouse | GAACCCCAAGGAAAAGAAGC | TGCTGTAGGTGAAGCGACTG |
| TIMP1 | Mouse | TCCCCAGAAATCAACGAGAC | ACCCCACAGCCAGCACTAT |
| TNF-α | Mouse | TCTCCAGCCACCAGCCCTCTAA | TGGCCATGGTAGGAGAAACAGG |
| IL-6 | Mouse | ACAAAGCCAGAGTCCTTCAGAG | GGCAGAGGGGTTGACTT |
| IL-1β IL-10 Arg-1 iNOS |
Mouse Mouse Mouse Mouse |
GGATGATGATGATAACCTGC AGCCTTATCGGAAATGATCCAGT CTCCAAGCCAAAGTCCTTAGAG GTTCTCAGCCCAACAATACAAGA |
CATGGAGAATATCACTTGTTGG GGCCTTGTAGACACCTTGGT AGGAGCTGTCATTAGGGACATC GTGGACGGGTCGATGTCAC |
| GAPDH | Mouse | AGGTCGTGTGAGGGATTG | TTAGTAGTAGTAGGGGGGGTCA |