Figure 3.

Mitral leaflets from patients with higher MR display proteomic change associated to proteolysis-, inflammatory-, and oxidative stress-related processes. (A) Comparison of proteome composition from the samples having moderately severe MR (MSMR n = 1) vs. the samples with severe MR (SMR n = 6) Volcano plot displaying the log2 fold-change (x-axis) against the statistical p-value (log scale) for all 845 proteins detected. Proteins with significantly increased expression (Log2FC > 0, p < 0.05) in SMR group are shown in red, while the proteins with significantly decreased expression (Log2FC > 0, p < 0.05) are expressed in blue. (B) Comparison of proteomic composition using linear correlation with MR level quantification (vena contracta). Dot plot displaying the R (x-axis) against the statistical p-value (log scale) for all 845 patients detected. Proteins with the significant and positive correlation (R > 0, p < 0.05) to MR are shown in red, while the proteins with significant and negative correlation (R < 0, p < 0.05) are presented in blue. (C) Venn diagram displaying modulated proteins obtain from the linear correlation to MR (orange) and MSMR vs. SMR comparison (purple), common proteins were identified in black. (D) Dot plot exhibiting p-value 845 patients identified from both analyses presented linear correlation to MR (orange), MSMR vs. MR comparison (purple), common proteins (black). (E) Heatmap of gene ontology biological process (GO:BP) enriched from protein significantly modulated.