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. 2021 Aug 12;8:688396. doi: 10.3389/fcvm.2021.688396

Figure 4.

Figure 4

Histo-cytometry assessment of MV cell composition correlated with echocardiography measures revealed that MR is concomitantly associated to EndMT and leaflet's thickness or tenting. (A) Representative MV section from patient with different range of cellular composition, (patients #14 upper and #12 bottom) stained with immunofluorescent co-staining of DAPI (blue), αSMA (green), CD45 (red), and CD31 (white), merge on the left panel (scale bar represents 100 μm). (B) Cell count per high power field (HPF) for αSMA single positive cells (αSMA+), CD45 single positive cells (CD45+), CD31 single positive cells (CD31+), along with CD31/αSMA double positive (CD31+ αSMA+) and CD31/CD45 double positive (CD31+ CD45+). (C) Shapiro-Wilk test of data distribution. (D) Univariable linear regression with VC. (E) Multivariable linear regression with VC. MV, mitral valves; VC, vena contracta; PL, posterior mitral leaflet; AL, anterior mitral leaflet; SP, single positive.