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. 2021 Aug 12;11:730288. doi: 10.3389/fcimb.2021.730288

Table 3.

Docking score and type of interaction of standard inhibitor and top three Azadirachta indica and Aloe barbadensis phytochemicals against SARS-CoV-2 Mpro, spike, and RdRp proteins.

Target PMID of ligand Ligand name Hydrogen-forming residues Residues with hydrophobic interaction
Mpro
Standard
92727 Lopinavir Asn142, Ser46, Thr45, Cys44, Thr25, Thr24, Leu27, Gly143, Thr26, Met49, Arg188, His41, Asp187, Val186, His164, Met165, Ser144, Phe140, Leu141, Cys145, His163, Glu166, Gln189,
Aloe
14889736 10-Hydroxyaloin A His41, Leu141, Gly143, Cys145, Leu27, Arg188, Thr26, Glu166, Ser144, His164, Asn142, His163, Thr25, Thr45, Cys44, Ser46, Met49, Gln189, Met165
158096 7-Hydroxyaloin B Leu141, His41, Gln189, Glu166, Thr190, Met49, Asn142, His163, Pro168, Met165, Arg188,
10648253 10-Hydroxyaloin B 6’-catate Gly143, His164, Cys145, Phe140, Ser46, Thr45, His41, Thr25, Cys44, Met49, Gln189, Met165, Leu141, Glu166, His163
Neem
10813969 Isoquercetin Glu166, Asn146, His41, Gly143, Leu27, Thr25, Cys44, Val42, Met49, Cys145, Met165, Arg188, Gln189, Thr190, Pro168, Leu167, Leu141, Ser144, His163, Phe140
97343-95-8 VEPAOL Thr26, Asn142, Gly143, Ser46, Gln189, Met49, His41, Leu27, His164, Met165, Glu166, His163, Ser144, Leu141, Phe140, HSI172, Cys145, Thr24, Thr25
29803-85-8 Nimbidiol Gly143, Thr26, Met49, Gln189, Ser46, Asn142, Thr24, Thr25, Leu27, Ser144, Cys145, His164, His41, Met165, Glu166
Spike
Standard
VE607 Asp422, Arg346, Thr345, Phe347, Arg509, Ser494, Lys417, Tyr421, Pro491, Leu492, Tyr351, Gln493, Ile418, Val350, Tyr495, Ile402, Ser349, Ala348, Phe497, Asp442
Aloe
100450 MS 3 Ser494, Gln493, Leu492, Pro491, Tyr421, Asn422, Lys417, Ile418, Tyr495, Val350, Gly496, Ser349
BB Phe497, Asp442, Phe347, Tyr495, Ser349, Val401, Ser443
12305761 Barbaloin Tyr495, Ser494, Val401, Gln493, Val350, Ser349
158096 7-Hydroxyaloin B Phe347, Asp442, Arg346, Val401, Tyr495, Val350, Ser494, Ser349, Phe497
Neem
10813969 Isoquercitin Phe347, Val350, Asp442, Phe497, Arg509, Arg346, Ala348, Val401, Ser349, Gln493, Tyr495, Tyr351, Ser494, Gln498, Pro499, Ser443
97343-95-8 Vepaol Val350, Asn422, Pro491, Tyr351, Gln493, Leu492, Lys417, Ile418, Tyr495, Ser349
RdRp
Standard
37542 Ribavirin Trp617, Asp618, Lys798, Glu811, Tyr619, Ser814, Phe812, Asp761, Asp760, Ala762, Gly616, Trp800, Cys799,
Aloe
158096 7-Hydroxyaloin B Asp618, Tyr619, Lys621, Asp760, Lys798, Glu811,
Asp761, Asp623, Cys622, Pro620, Lys551, Arg553, Ser814
6857486 CHEBI:35671 Asp760, Asp761, Ala762, His810, Glu811, Asp618, Tyr619, Lys798, Cys799, Trp800, Gly616, Trp617, Val763, Phe812, Ser759, Ser814
5317657 UNII-73899319HU Arg553, Ser759, Asp760, Ser814, Lys798, Lys545, Glu811, Phe812, Trp800, Cys799, Trp617, Tyr619, Trp617, Cys813, Asp618, Asp761, Leu758,
Neem
10813969 Isoquercetin Trp617, Asp618, Tyr619, Asp760, Lys798, Pro620, Lys621, Cys622, Cys799, Trp800, Gly616, Ala762, Asp761, Glu811, Ser814, Ser759
184310 Isonimocinolide Tyr619, Lys621, Asp761, Ser814, Asp618, Lys798, Asp623, Cys622, Pro620, Leu758, Asp760, Ser759, Cys813, Ala762, Phe812, Glu811, Trp617, Gly616, Trp800

BB- (10R)‐2,8‐dihydroxy‐6‐(hydroxymethyl)‐1‐methoxy‐10‐[(2R,3R,4R,5R,6R)‐3,4,5‐trihydroxy‐6‐(hydroxymethyl)oxan‐2‐yl]‐9,10‐dihydroanthracen‐9‐one.

The amino acids shown in bold were involved in hydrogen bond formation with their respective protein.