Fig. 1.

Immunocytochemistry of CPT1 and CEPT1 in HEK293 cells. HEK293 cells were transfected with the expression vectors for CPT1 (A, B) or CEPT1 (C) tagged with HA at the C terminus. After fixation, the cells were permeabilized with 0.1% Triton X-100 (w/v), immunostained with anti-HA mouse antibody, then with anti-mouse IgG Alexa Fluor 488 (green). The cis- and trans-Golgi were stained with anti-GM130 and anti-TGN46 rabbit antibodies, respectively, followed by antirabbit IgG Alexa Fluor 594 (red). The ER was visualized using DsRed-ER (red). Nuclei were stained with DAPI (blue). Arrows indicate perinuclear Golgi-like structures. Arrowheads indicate dot-like structures. Bars indicate 10 μm.