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. 2021 Aug 11;22(4):735. doi: 10.3892/ol.2021.12996

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Copyright: © Ikari et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — RHOA expression in digestive tract cancer cell lines. The expression of RHOA in digestive tract cancer cell lines was detected by immunoblotting using a rabbit monoclonal anti-RHOA antibody. β-actin was used as a loading control. The pictures were cropped from the same blot probed with the anti-RHOA antibody firstly (upper) and the anti-β-actin antibody subsequently (lower). The experiments were performed twice and similar results were obtained. GCIY, HGC-27, KATO III, MKN1 and MKN45 harbored wild-type RHOA. RHOA mutations were detected as p.Glu64del in AGS, p.Arg5Trp and p.Phe39Leu in SNU16, p.Ala61Val in SNU719, p.Tyr42Ser in OE19, p.Arg5Gln, p.Tyr42Cys in CCK-81 and p.Gly17Glu in SW948. RHOA, ras homolog family member A.