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. 2021 Aug 16;22(4):737. doi: 10.3892/ol.2021.12998

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Overexpression of TAGLN2 enhances the expression of STAT3 downstream of ANXA2. (A) ANXA2 was predicted to be associated with STAT3 according to the Search Tool for the Retrieval of Interacting Genes/Proteins database. After overexpressing TAGLN2, the expression of ANXA2 was measured using (B) RT-qPCR and (C) western blotting. After knocking down ANXA2 in TAGLN2-overexpressing cells, the expression of ANXA2 was detected by (D) RT-qPCR and (E) western blotting. (F) Western blotting was used to examine the expression of STAT3 and p-STAT3. ***P<0.001. RT-qPCR, reverse transcription-quantitative PCR; TAGLN2, transgelin 2; ANXA2, annexin 2; STAT3, signal transducer and activator of transcription 3; sh, short hairpin; NC, negative control; OV, overexpressing plasmid; p, phosphorylated.