Figure 3.

Lanatoside C induces HeLa cell apoptosis. (A) HeLa cells were treated with lanatoside C for 24 h and Annexin-V/PI staining with flow cytometry detection was performed. (B) Quantification analysis of apoptotic rates in lanatoside C-treated HeLa cells was performed. (C) Expression of apoptosis-related proteins, cleaved caspase-9, cleaved caspase-3 and cleaved PARP was detected via western blotting. β-actin served as the loading control. The expression levels of (D) cleaved caspase-9, (E) cleaved caspase-3 and (F) cleaved PARP were quantified using ImageJ software. Data are presented as the mean ± SEM (n=3). *P<0.05; **P<0.01; ***P<0.001. LC, lanatoside C.