Figure 4.

Lanatoside C decreases MMP and increases cellular ROS production in HeLa cells. (A) Following treatment with lanatoside C for 24 h, MMP was detected via JC-1 staining and flow cytometry in HeLa cells. (B) JC-1 polymer in HeLa cells was analyzed to determine quantitative changes in the loss of MMP. (C) Cells were treated with lanatoside C at 2.5 µM for 4 or 6 h, and cellular ROS production in HeLa cells was monitored via DCFH-DA probe with fluoresce microscopy detection. (D) Statistical analysis of ROS production (MFI, mean fluorescence intensity) in HeLa cells using ImageJ software. Data are presented as the mean ± SEM (n=3). *P<0.05; **P<0.01; ***P<0.001. MMP, mitochondria membrane potential; ROS, reactive oxygen species; LC, lanatoside C.