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Generation of the NEUROG3-HA-P2A-Venus hiPSC line by CRISPR/Cas9. (A) CRISPR/Cas9-mediated targeting of the NEUROG3 locus to knock in the 3HA-P2A-3NLS-Venus cassette in fusion with NEUROG3 coding sequence. Position of the sgRNA and the primers used for genotyping and sequencing is indicated. (B). Genotyping by PCR using the indicated primers. The clone used throughout this study is clone#31. See Suppl. Table 1 for oligonucleotide sequences.
