Supplemental Figure 5.

Validation of selected NEUROG3 targets by Luciferase assays. Sequences encompassing NEUROG3-bound regions of MLXIPL, ETS2, and ISX genes were cloned into pGL4.23-[luc2/minP] vector. Luciferase activity was assessed in HEK293T cells by transfection of reporter constructs with pcDNA3-NEUROG3-3HA-P2A-3NLS-Venus (+NEUROG3) or pcDNA3 empty vector (no NEUROG3) and Renilla luciferase expressing plasmid for normalization. Results are expressed as relative activity to the “no NEUROG3” condition and shown as mean ± SD (n = 6). Two-way ANOVA followed by Turkey's multiple comparisons tests were performed for statistical significance. Non significant = ns, p > 0.0001 = ∗∗∗∗.