FIG. 1.
FLP-OUT system. (A) hsp70:FLP construct in the FLP1 transgenic line. (B) lacZ-catalase-Cu/ZnSOD expression constructs before recombination. (C) Expression constructs after FLP/FRT-mediated recombination. In the catalase and Cu/ZnSOD expression constructs, the transcriptional stop sequence is the 3′ end and polyadenylation signal sequence of the hsp70 gene, while in the lacZ expression construct the stop sequence is the Draf gene (53). (D) β-gal enzyme levels were assayed in extracts of adult Drosophila of the indicated genotypes. The data are the mean and standard deviation of triplicate assays. Statistically significant differences (P < 0.05) between HP and Co were determined with two-sided t tests and are indicated by an asterisk.