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. 1999 Jan;19(1):229–240. doi: 10.1128/mcb.19.1.229

TABLE 3.

Mapping of KSR1 sites phosphorylated by MAPK in vitro

HPLC fraction(s) Edman degradationb Phosphoamino acid(s) Amino acid residues spanned by deletion mutant Site identified (sequencea)
37–38 6 T 249–320 T274 (LKPPRT*PPPPSR)
53 11 T 249–320 T260 (ALHSFITPPTT*PQLR)
59 S, T 424–539 S443?
61–62 S, T 424–539 S443?
65–66 S, T 249–320 ?
a

Asterisks indicate sites of phosphorylation. 

b

Cycle in which 32P counts per minute were released.