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Tryptic HPLC profiles of in vitro 32P-labeled KSR1 mutant proteins
| Construct | HPLC 32P profile |
|---|---|
| WT KSR1 | Contains major peaksa in fr.b 37–38, 53, 59, 61–62, and 65–66 |
| T260A-KSR1 | Missing peak in fr. 53 |
| T274A-KSR1 | Missing peak in fr. 37–38 |
| S443A-KSR1 | Missing peak in fr. 59, and 61–62 |
| T274A,S443A-KSR1 | Missing peak in fr. 37–38, 59, and 61–62 |
| T260A,S274A,S443A-KSR1 | Missing peak in fr. 37–38, 53, 59, and 61–62 |
Containing >500 cpm.
fr., fraction.
