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Indispensability of arginine finger for ATP binding. Phosphate imaging of 32P-ATP and different gp16-eGFP mutants. The arginine mutant R146A loses the ATP binding ability completely. The WT, Walker B motif (118/9DE), and arginine finger mutant (R146A) were incubated with 40bp DNA, pRNA, and 10 μM 32P-ATP with (+) and w/o (−) γ-S-ATP. “0” is the negative control without gp16. (A) Phosphate imaging overnight showing signal of 32P-ATP. (B) eGFP channel showing the signal of gp16-eGFP.
