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. 2021 Jul 29;15(8):13260–13266. doi: 10.1021/acsnano.1c02973

Figure 3.

Figure 3

Indispensability of arginine finger for DNA binding. The interaction of DNA labeled with Alexa647 with either the WT gp16 or the Walker A–arginine finger double mutant gp16 (WA-RF gp16) were tested by electrophoretic mobility shift assay (EMSA). When increasing the amount of WT gp16, more DNA bound to the WT gp16 protein (lane 2–7), while the Walker A–arginine finger mutant did not show binding to DNA (lanes 8–13). (A) eGFP channel showing the signal of gp16-eGFP. (B) Alexa647 channel showing the signal of red fluorescent-DNA. (C) Overlap image (merged) of A and B.