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. 2021 Jul 29;15(8):13260–13266. doi: 10.1021/acsnano.1c02973

Figure 5.

Figure 5

Indispensability of arginine finger for DNA packaging. The arginine finger mutant (R146A) was biologically inactive in DNA packaging as demonstrated by its inhibition to DNA packaging activity of WT gp16. An in vitro DNA packaging assay was carried out using a series of titrations of WT gp16 with buffer, Walker B mutant E119A, and arginine finger mutant R146A. (A) EtBr staining of the agarose gel showing the packaged DNA (black arrow). R146A gp16 by itself did not have DNA packaging activity. (B) Bar chart of the grayscale measurement of the packaged DNA. Data were normalized to the activity of only WT gp16.