FIGURE 4.

Evaluation of LOX‐1⁺ cells using multiplex immunofluorescence in CRC tissues. A, MDSC markers, CD11b (red), CD15 (green), and LOX‐1 (magenta). Cells co‐expressing CD11b⁺ CD15⁺ are in yellow. Some LOX‐1⁺ stromal cells partially express CD11b⁺ and CD15⁺ cells (white arrows). B, LOX‐1⁺ stromal cells expressed CD45 (green) but did not express α‐SMA (magenta). C, Almost LOX‐1⁺ cells expressed CD163 (green). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. CRC, clinical colorectal cancer; LOX‐1, lectin‐like oxidized low‐density lipoprotein receptor‐1; MDSCs, polymorphonuclear MDSCs