Table 2.
Gene-specific primer sequences of tight junction protein and inflammatory cytokine genes in the ileal tissue.
| Items 1 | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| CLDN1 | AGAAGATGCGGATGGCTGTC | CCCAGAAGGCAGAGAGAAGC |
| CLDN2 | TCCTCCCTGTTCTCCCTGATAG | CCTTGCAGTGGGCAGGAA |
| CLDN3 | GATGCAGTGCAAAGTGTACGA | GTCCTGCACGCAGTTGGT |
| CLDN4 | TATCATCCTGGCCGTGCTA | CATCATCCACGCAGTTGGT |
| OCLN | GGAGTGATTCGGATTCTGTCTATGCT | CGCCTGGGCTGTTGGGTTGA |
| MUC1 | CCCTGGCCATCATCTATGTC | TGCCCACAGTTCTTTCGTC |
| IL-1β | GCCCTGTACCCCAACTGGTA | CCCAGGAAGACGGGCTTT |
| IL-6 | GCGCAGCCTTGAGGATTTC | CCCAGCTACATTATCCGAATGG |
| INF-γ | GAGCCAAATTGTCTCCTTCTAC | CGAAGTCATTCAGTTTCCCAG |
| TNF-α | CTTGGGTTTGGATTCCTGGAT | CTTCCCTGGCAGCCACAT |
| MCP1 | TCCCACACCGAAGCTTGAAT | CACAGGAGGGCTGCAGAGA |
| 18S rRNA | GGCTACCACATCCAAGGAAG | TCCAATGGATCCTCGCGGAA |
1 CLDN1 = claudin-1; CLND2 = claudin-2; CLDN3 = claudin-3; CLDN4 = claudin-4; OCLN = occludin; MUC1 = mucin-1; IL-1β = interleukin-1β; IL-6 = interleukin-6; INF-γ = interferon-γ; TNF-α = tumor necrosis factor-α; MCP1 = monocyte chemoattractant protein-1.