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. 2021 Jul 29;11(8):2234. doi: 10.3390/ani11082234

Table 2.

Primers used for quantitative PCR analysis.

Target Bacterial
Populations
Primers Sequence (5′–3′) Reference
Total bacteria UniF CCTACGGGAGGCAGCAG [32]
UniR ATTACCGCGGCTGCTGG
Firmicutes Firm350f GGCAGCAGTRGGGAATCTTC [33]
Firm814r ACACYTAGYACTCATCGTTT
Bifidobacterium spp. Bif_F TCGCGTCYGGTGTGAAAG [34]
Bif_R CCACATCCAGCRTCCAC
Enterococcus spp. Ent_F CCCTTATTGTTAGTTGCCATCATT [34]
Ent_R ACTCGTTGTACTTCCCATTGT
Lactobacillus spp. Lac_F AGCAGTAGGGAATCTTCCA [35]
Lac_R CACCGCTACACATGGAG
Clostridium cluster I CloI-F TACCHRAGGAGGAAGCCAC [36]
CloI-R GTTCTTCCTAATCTCTACGCAT
Escherichia coli Coli_F GTTAATACCTTTGCTCATTGA [37]
Coli_R ACCAGGGTATCTAATCCTGTT
Faecalibacterium prausnitzii Fprau 07 CCATGAATTGCCTTCAAAACTGTT [38]
Fprau 02 GAGCCTCAGCGTCAGTTGGT