Table 2.

Primer sequences used in RT-qPCR reaction.

Gene	Name	Primer Sequences 1	References
ACTB	Actin beta	F: CACAGATCATGTTTGAGACCTT R: CATCACAATACCAGTGGTACG	[25]
G6PDH	Glucose-6-phosphate dehydrogenase	F: CGGGAACCAAATGCACTTCGT R: GGCTGCCGTAGAGGTATGGGA	[25]
IFNG	Interferon gamma	F: ACACTGACAAGTCAAAGCCGC R: AGTCGTTCATCGGGAGCTTG	[26]
IL1B	Interleukin 1 beta	F: GGAGGTTTTTGAGCCCGTC R: TCGAAGATGTCGAAGGACTG	[21]
IL10	Interleukin 10	F: CATGCTGCTGGGCCTGAA R: CGTCTCCTTGATCTGCTTGATG	[27]
IL12	Interleukin 12	F: TTGCCGAAGAGCACCAGCCG R: CGGTGTGCTCCAGGTCTTGGG	[26]
IL2	Interleukin 2	F: GCTTATGGAGCATCTCTATCATCA R: GGTGCACTCCTGGGTCTC	[28]
IL4	Interleukin 4	F: GCTCTCAGTGCCGCTGATG R: GGAAACCTCTCCCTGGATGTC	[29]
IL17	Interleukin 17	F: CCGTCTTCTGCTGAGAGGAGTG R: ACCGTTGTTCCGTCCCATCAC	[28]
TNFAIP6	Tumor necrosis factor-inducible gene 6 protein	F: CTGGCTGTCCCTGTGTGATT R: TCAGGTGCTATTGCTGCGAG	This study
NCF1C	Neutrophil Cytosolic Factor 1C	F: CTGTGGATGGTGTCACCGAA R: TGCCATTCTCACAGCCCTAC	This study
AvBD1	Avian beta-defensin 1	F: AAACCATTGTCAGCCCTGTG R: TTCCTAGAGCCTGGGAGGAT	[21]
CATHL2	Cathelicidin	F: AGGAGAATGGGGTCATCAGG R: GGATCTTTCTCAGGAAGCGG	[21]
MUC6	Mucin 6	F: TTCAACATTCAGTTCCGCCG R: TTGATGACACCGACACTCCT	[21]
CLDN1	Claudin 1	F: TCTTCATCATTGCAGGTCTGTC R: AACGGGTGTGAAAGGGTCAT	[21]
TJAP1	Tight junction-associatedprotein 1	F: AGGAAGCGATGAATCCCTGTT R: TCACTCAGATGCCAGATCCAA	[21]
FFAR2	Free fatty acid receptor 2	F: GCTCGACCCCTTCATCTTCT R: ACACATTGTGCCCCGAATTG	[21]
FFAR4	Free fatty acid receptor 4	F: AGTGTCACTGGTGAGGAGATT R: ACAGCAACAGCATAGGTCAC	[21]
GLUT1	Glucose transporter 1	F:AGATGACAGCTCGCCTGATG R:GTCTTCAATCACCTTCTGCGG	[21]

Notes: ¹ F, forward primer; R, reverse primer.