TABLE 1.
Specific transient expression of various rodent pro-α1(I) collagen promoter-CAT constructs in Rat-1, v-fos-transformed 1302-4-1, and revertant EMS-1-19 cell lines
| Construct | Promoter activitya (pg of CAT/plasmid copy no. [107])
|
||
|---|---|---|---|
| Rat-1 | v-fos transformants | Revertant | |
| pColCAT3.5b | 0.7 ± 0.2 | NDd | ND |
| pColCAT2.3c | 9.8 ± 0.5 | 1.7 ± 0.2 | 5.0 ± 0.3 |
| pColCAT0.9b | 4.7 ± 0.4 | 1.0 ± 0.2 | 2.3 ± 0.2 |
| pColCAT0.2c | 18.1 ± 2.5 | 3.2 ± 0.3 | 21.2 ± 3.6 |
Data are directly related to the samples used for Fig. 4. Transfected DNA and CAT enzyme determinations are described in Materials and Methods. Data are expressed as mean ± standard error of the mean for three determinations.
Rat promoter.
Mouse promoter.
ND, not detected.