Table 1.
Primers used for real-time PCR, annealing temperature (Ta), and amplicon length.
| Gene Symbol |
Accession Number |
Nucleotide Sequence of Primers, 5′-3′ | Ta (°C) |
Product Length (bp) |
|---|---|---|---|---|
| HPRT1 | DQ178126 | Forward: CCGAGGATTTGGAAAAGGT Reverse: CTATTTCTGTTCAGTGCTTTGATGT |
60 | 181 |
| YWHAZ | DQ178130 | Forward: ATGCAACCAACACATCCTATC Reverse: GCATTATTAGCGTGCTGTCTT |
60 | 178 |
| GADPH | NM_001206359.1 | Forward: TGGTGAAGGTCGGAGTGAAC Reverse: GAAGGGGTCATTGATGGCGA |
60 | 104 |
| TRPA1 | XM_021089237.1 | Forward: GAATTTACTCATTGGTTTGGCAGTTGGTG Reverse: CGGTGATGGATTTCTGATCGACCTTG |
58 | 155 |
| TRPV1 | XM_013981216.2 | Forward: GGACAGCGAGTTCAAAGACC Reverse: CCGTTTTCCACCAGAAGTGT |
63 | 240 |
| CCK | XM_021068544.1 | Forward: CAGGCTCGAAAAGCACCTTC Reverse: GCGGGGTCTTCTAGGAGGTA |
60 | 157 |
| GLP-1 | XM_005671882.3 | Forward: AGAACTCCGCCGCAGACA Reverse: TAAAGTCTCGGGTGGCAAGATT |
60 | 83 |