. Author manuscript; available in PMC: 2021 Aug 26.

Published in final edited form as: ACS Chem Biol. 2019 Nov 6;14(12):2576–2584. doi: 10.1021/acschembio.9b00429

Table 1.

Hydrolase activities of ARH and Macrodomain protein families

Proteins	Substrates
ARHs	α-NAD⁺	OAADPr	ADP-ribosyl-arginine	MAR	PAR	β-NAD⁺	DNA	RNA
ARH1	yes*⁺	yes^a	yes^d	ADPr-Gas^f	yesⁱ	no*⁺	noⁿ	no^o
ARH3	yes*⁺	yes^a	no^e	ADPr-serine^m	yes^e	no*⁺	yesⁿ	yes^o
Macrodomains
MacroD1	yes*	yes^b	no*	ADPr-PARP10^g	no^g	no*	---	yes^o
MacroD2	yes*	yes^b	no*	ADPr-PARP10^g	no^g	no*	yesⁿ	yes^o
C6orf130	yes*	yes^c	no*	ADPr-PARP10^g	no^j	no*	yesⁿ	yes^o
Af1521	yes*	---	no*	ADPr-PARP10^g	no^k	no*	---	---
PARGCD	no*⁺	---	---	no^h	yes^l	---	yesⁿ	yes^o

ADPr-PARP10, mono-ADP-ribosylated PARP10; ADPr-Gαs; Gαs modified on arginine; OAADPr, O-acetyl-ADP-ribose; MAR, mono-ADP-ribose; PAR, poly-ADP-ribose. “yes or no, indicates activity has been shown for the respective substrate, (*) indicates this manuscript, (⁺) magnesium in the assay. Dashes (----) indicate those activities that have not been reported, a³⁵, b⁴¹, c⁴⁷, d⁵, e²⁵, f⁵⁰, g⁴⁰, h⁴⁰, i²⁵, j⁵¹, k⁴⁸, l²⁵, m⁴,n⁷,o⁸.