(A) Representative pictures showed that subarachnoid blood clots mainly presented around the circle of Willis in the rat brain at 24 h after SAH; (B) SAH grading scores of all SAH groups. (C) Representative western blot bands of KISS1, GPR54 and ARRB2; (D) Time course and densitometric quantification of KISS1, GPR54 and ARRB2. *p < 0.05 vs. Sham group. Data was represented as mean ± SD, n = 6 per group, one-way ANOVA Tukey test was used for the comparison between sham and SAH groups. (E) Immunofluorescence staining of GPR54 or ARRB2 with neurons (NeuN, red), microglia (Iba-1, red) and astrocytes (GFAP, red) at 24 h after SAH. The small black box in the coronal brain slice (left of top panel) indicated the area where microphotograph was taken. Scale bar = 50μm, n = 2 for each group.