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. Author manuscript; available in PMC: 2022 Jul 6.
Published in final edited form as: Anal Chem. 2021 Jun 24;93(26):9119–9128. doi: 10.1021/acs.analchem.1c00847

FIGURE 2.

FIGURE 2

LC-MS analysis of intact protein standard mixture with spectra taken from the apex of elution profiles for both protein AG (50 kDa) and exo-Klenow fragment (68 kDa). A) Spectrum of protein AG under normal MS1 conditions with the most abundant charge state highlighted in red. B) Spectrum of protein AG under PTR-MS1-PIP conditions with the most abundant charge state highlighted in red and the range of m/z intended for PIP shaded in grey. C) LC-MS total-ion chromatogram for the analysis with the region over which protein AG elutes highlighted in blue. Figures 2D, E, & F the same respectively for the exo-Klenow fragment.