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. Author manuscript; available in PMC: 2022 Jul 6.
Published in final edited form as: Anal Chem. 2021 Jun 24;93(26):9119–9128. doi: 10.1021/acs.analchem.1c00847

FIGURE 3.

FIGURE 3

LC-MS analysis of whole cell lysate proteins from H. Sapiens MCF7 cells. A) Region of mass spectrum taken with normal MS1 conditions containing isotopic peak clusters for a protein exhibiting a “family” of associated proteoforms. B) The same region measured under PTR-MS1-PIP conditions shows a more defined set of associated proteoforms from improved S:N. C) Deconvolution of the peak clusters shown in the normal MS1 spectrum yielded 5 proteoforms. D) Deconvolution of the respective peak clusters of the PTR-MS1-PIP spectrum yielded 15 proteoforms. Xtract-determined monoisotopic mass differences (Δm, Da) among proteoforms are indicated.