Table 6.

Median PCR and ELISA parameters obtained at 3 and 9 days for infected and controls.

Time	Method	Parameter	Day 3			Day 9			Significance
			Median	Min	Max	Median	Min	Max	p > t
Infected	Real-Time PCR 1	MHC-I (lymph nodes)	42.34	12.86	42.34	65.79	0.07	176.07	0.4969
		MHC-II (lymph nodes)	0.46	0.25	0.66	0.735	0.01	1.31	0.0894
		IFN-γ (jejunum)	6.80	0.03	213.04	0.89	0.10	36.89	0.3082
		IL-1β (jejunum)	0.51	0.45	0.81	0.63	0.17	1.23	0.7336
		TLR2 (jejunum)	1.19	0.25	15.94	0.53	0.11	2.58	0.2345
		TLR4 (jejunum)	0.51	0.48	2.35	0.49	0.17	1.62	0.4439
	ELISA	IgA serum (mg/mL)	0.51	0.12	0.57	0.19	0.10	0.70	0.3436
		IgA scrape (µg/mL)	99.44	98.32	156.01	98.16	96.83	104.22	0.3949
		CXC9L scrape (ng/mg TP)	0.96	0.15	2.88	1.98	0.66	4.55	0.1742
		TNF-α scrape (ng/mg TP)	0.37	0.07	1.62	0.72	0.35	1.15	0.3494
		IL_8 scrape (ng/mg TP)	3.33	0.25	4.55	3.42	2.11	5.19	0.7341
		IL-1 scrape (pg/mL)	0.07	0.01	0.21	0.07	0.02	0.12	0.8649
Controls	Real-Time PCR 1	MHC-I (lymph nodes)	13.27	13.04	13.50	5.80	0.23	37.40	0.3545
		MHC-II (lymph nodes)	0.65	0.51	0.78	0.72	0.00	1.62	1.0000
		IFN-γ (jejunum)	11.42	1.36	21.48	0.47	0.04	4.27	0.1649
		IL-1β (jejunum)	0.57	0.12	1.01	0.53	0.37	0.76	1.0000
		TLR2 (jejunum)	0.78	0.58	0.98	0.40	0.36	0.79	0.1649
		TLR4 (jejunum)	1.09	0.36	1.82	0.41	0.38	0.51	1.0000
	ELISA	IgA serum (mg/mL)	0.57	0.54	0.59	0.23	0.16	0.39	0.0641
		IgA scrape (µg/mL)	98.94	96.67	101.20	105.49	99.89	112.95	0.3545
		CXC9L scrape (ng/mg TP)	1.10	0.98	1.22	2.20	1.56	2.84	0.0641
		TNF-α scrape (ng/mg TP)	0.34	0.31	0.37	0.81	0.57	0.98	0.0641
		IL_8 scrape (ng/mg TP)	1.74	1.69	1.79	3.22	1.49	4.31	0.3545
		IL-1 scrape (pg/mL)	0.06	0.06	0.06	0.11	0.06	0.16	0.1336

¹ Data were normalized to beta-actin expression and reported as relative expression. TP: Total protein. The comparisons were performed using the Wilcoxon Rank Sum test for independent groups.