Table 1.
Primers for real-time PCR analyses.
| Gene | Primer Sequence, 5′ to 3′ | Accession Number | Product Size (bp) |
|---|---|---|---|
| MCT4 | F: GTTTGGGATAGGCTACAGTGACACA | NM_001109980.1 | 106 |
| R: GCAGCCAAAGCGATTCACA | |||
| NHE1 | F: CCTCTACAGCTACATGGCCTAC | Etschmann et al. (2006) | 113 |
| R: GGGAGATGTTGGCTTCCA | |||
| PAT1 | F: CCTTGAGGCACGGCTAC | BC123616.1 | 123 |
| R: GCACCAGACTCCGAGACATA | |||
| AE2 | F: AGCAGCAACAACCTGGAGT | NM_001205664.1 | 123 |
| R: GGTGAAACGGGAGACGAA | |||
| vH+ ATPas | F: TTTTATTGAACAAGAAGCCAATGA | Albrecht et al. (2008) | 182 |
| R: GATTCATCAAATTGGACATCTGAA | |||
| IL-1β | F: GAAGAGCTGCACCCAACA | XM_013967700.2 | 172 |
| R: CAGGTCATCATCACGGAAG | |||
| IL-6 | F: AGATATACCTGGACTTCCT | NM_001285640.1 | 80 |
| R: TGTTCTGATACTGCTCTG | |||
| TNF-α | F: TGGTTCAGACACTCAGGT | NM_001286442.1 | 75 |
| R: CGCTGATGTTGGCTACAA | |||
| CXCL8 | F: TGTGTGAAGCTGCAGTTCTGT | XM_005681749 | 186 |
| R: TGGGGTCTAAGCACACCTCT | |||
| CCL5 | F: GTCTGCCTCCCCATATGCCTC | XM_005693201 | 187 |
| R: CTCTCGCACCCACTTCTTCTC | |||
| CXCL6 | F: CCAAGGTGGAAGTGGTAGCC | XM_005681937 | 149 |
| R: CTGGGCAATTCTTCCAACGC | |||
| TLR-2 | F: TTGACAAGAAGGCCATCCCC | NM_001285603 | 105 |
| R: AGAACGCTTCCTGCTGAGTC | |||
| TLR-4 | F: TTCAACCGTATCACGGCCTC | NM_001285574 | 127 |
| R: TGACCCACTGCAGGAAACTC | |||
| MyD88 | F: TTGAGAAGAGGTGCCGTCG | XM_013973392 | 187 |
| R: CAGACAGTGATGAAGCGCAG | |||
| Tollip | F: CGACGTAGGCTTAGCGTGAA | XM_013976999 | 142 |
| R: CTGGTCTCACGCATCTACCG | |||
| IRF3 | F: TTGTGAACTCAGGGGTCAGG | XM_013971473 | 125 |
| R: TGGGCTCAAGTCCATGTCAC | |||
| IFIT3 | F: AAATTCTGAGGCAGGCCGTT | XM_005698196 | 127 |
| R: TTTCCCAGAGCCTCGACAAC | |||
| NF-κB | F: CTGGAAGCACGAATGACAGA | XM_005681365 | 197 |
| R: GCTGTAAACATGAGCCGTACC | |||
| GAPDH | F: CAAAGTGGACATCGTTGCCA | XM_005681365 | 197 |
| R: TGGAAGATGGTGATGGCCTT |
F, forward; R, reverse.