Table 2.

Sequences of primers used for real-time PCR.

Target	Forward Primer	Reverse Primer	Reference
Total bacteria	5′-CGGCAACGAGCGCAACCC-3′	5′-CCATTGTAGCACGTGTGTAGC-3′	[34]
Fibrobacter succinogenes	5′ GTTCGGAATTACTGGGCGTAAA 3′	5′ CGCCTGCCCCTGAACTATC 3′	[35]
Ruminococcus flavefaciens	5′ CGAACGGAGATAATTTGAGTTTACTTAGG 3′	5′ CGGTCTCTGTATGTTATGAGGTATTACC 3′	[34]
Ruminococcus albus	5′ CCCTAAAAGCAGTCTTAGTTCG 3′	5′ CCTCCTTGCGGTTAGAACA 3′	[36]
Fungi	5′-GAGGAAGTAAAAGTCGTAACAAGGTTTC-3′	5′-CAAATTCACAAAGGGTAGGATGATT-3′	[34]
Protozoa	5′-GCTTTCGWTGGTAGTGTATT-3′	5′-CTTGCCCTCYAATCGTWCT-3′	[35]