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. 2021 Aug 17;11(8):2422. doi: 10.3390/ani11082422

Figure 5.

Figure 5

Immunostaining for LIN28 (marker for PGCs, A–H) and CD117 (marker for pluripotent cells, I–L) in female (A–D,G,H,K,L) and male (E,F,I,J) gonads from 45-day-old horse fetus (A–C) and 60-day-old horse fetuses (D–L). (B) Magnified image of area identified in (A). (C) Magnified image of area identified in (B). Inserts in F, H, J and L represent negative controls. At 45 days of development, PGCs were presumably migrating from the mesonephros to the gonad, but this was not the case at 60 days. PGCs were restricted to the tubular structures in males (E,F) and organized in cord-like structures localized in the cortical region in females (G,H). CD117 showed a similar distribution pattern to LIN28 (I–L). Scale bars in (A) and (D): 200 µm; all other pictures: 50 µm.