Figure 6.

Immunostaining for ß-catenin (A), pan-cytokeratin (B), vimentin (C) and laminin (D) in Wolffian (Wd) and Müllerian (Md) ducts. Representative images from a 60-day-old female horse fetus are presented. Both ducts were positive for ß-catenin and vimentin irrespective of fetal sex (A,B). While the mesonephric ducts consistently stained positive for cytokeratin in all samples, in the paramesonephric ducts the staining ranged from only apical cytoplasmic staining to an entirely positive cytoplasm (C). Both ducts presented a well-developed basal lamina in both sexes (D). Scale bar for all pictures: 50 µm.