Table 1.
List of antibodies used for immunohistochemistry and immunofluorescence.
| Antibody | Clone | Host | Raised against (Species) | Manufacturer | Catalog # | Dilution | Target |
|---|---|---|---|---|---|---|---|
| AMH | polyclonal | Goat | human | Santa Cruz, Dallas, TX, USA | Sc-6886 | 1:200 | Sertoli cells |
| LIN28 | polyclonal | Rabbit | human | Abcam, Cambridge, UK | Ab46020 | 1:15.000 | PGCs |
| Ki67 | 8D5 | Mouse | human | Cell Signaling, Danvers, MA, USA | 9449 | 1:400 | Proliferating cells |
| CD117 | polyclonal | Rabbit | human | Dako, Santa Clara, CA, USA | A4502 | 1:600 | Pluripotent cells |
| Vimentin | V9 | Mouse | pig | Dako | M0725 | 1:500 | Mesenchyme–endothelium |
| Laminin | polyclonal | Rabbit | rat | Dako | Z0097 | 1:10.000 | Basal lamina |
| Pan Cytokeratin | AE1 + AE3 | Mouse | human | Cell Marque, Rocklin, CA, USA | 313M-16 | 1:500 | Epithelium–cytoskeleton |
| ß-catenin | 9G2 | Mouse | human | Acris, Herford, Deutschland | AM00020PU-N | 1:400 | Cell–cell adhesion |
| Tyramide Triple Immunofluorescence | |||||||
| LIN28 | polyclonal | Rabbit | human | Abcam | Ab46020 | 1:1000 | PGCs |
| CD117 | polyclonal | Rabbit | human | Dako | A4502 | 1:100 | Pluripotent cells |
| Ki67 | 8D5 | Mouse | human | Cell Signaling | 9449 | 1:200 | Proliferating cells |