. 2021 Aug 18;11(8):2425. doi: 10.3390/ani11082425

Table 2.

Effect of supplementary feeds on alpha diversity of rumen bacteria in grazing, lactating yaks.

Alpha Diversity Indices	CON	RPA	C	RPA+C	Overall Data ¹	SEM ²	p-Values ³
Alpha Diversity Indices	CON	RPA	C	RPA+C	Overall Data ¹	SEM ²	RPA	C	RPA × C
Sobs	1839	1827	1713	1792	1793	46.4	0.552	0.052	0.243
Shannon	6.23	6.22	6.02	6.26	6.18	0.044	0.195	0.352	0.163
Simpson	0.0047	0.0047	0.0054	0.0045	0.0048	0.0009	0.364	0.626	0.309
ACE	2117	2106	2003	2048	2069	21.3	0.705	0.046	0.472
Chao	2141	2129	2029	2077	2094	22.5	0.694	0.079	0.486
Coverage, %	99.1	99.1	99.0	99.1	99.1	0.0003	0.984	0.604	0.270

CON, control group; RPA, with supplementary 15 g/day RP-Lys and 5 g/day RP-Met; C, with supplementary 1.2 kg/day concentrate; RPA+C, with supplementary 15 g/day RP-Lys and 5 g/d RP-Met and 1.2 kg/day concentrate. ¹ Sequences obtained from all 24 ruminal fluid samples (average of four treatments); ² SEM, standard error of mean; n = 6 for each group; ³ RPA = effect of rumen-protected amino acids; C = effect of concentrate; RPA × C = interaction between concentrate and rumen-protected amino acids.