. 2021 Aug 18;11(8):2425. doi: 10.3390/ani11082425

Table 3.

Relative abundances (percentage of total reads) of rumen bacteria at phylum level in grazing, lactating yaks offered supplementary feeds.

Items	CON	RPA	C	RPA+C	Overall Data ¹	SEM ²	p-Value ³
Items	CON	RPA	C	RPA+C	Overall Data ¹	SEM ²	RPA	C	RPA × C
Bacteroidetes	64.3	61.4	62.6	57.6	61.5	2.84	0.275	0.251	0.663
Firmicutes	26.2	29.5	27.2	34.4	29.3	2.80	0.164	0.150	0.322
Tenericutes	2.82	3.33	3.60	2.59	3.09	0.476	0.612	0.973	0.144
Spirochaetes	2.05	1.98	2.36	1.43	1.96	0.411	0.254	0.775	0.318
Patescibacteria	2.03	1.53	1.31	1.34	1.55	0.231	0.333	0.078	0.281
Proteobacteria	1.36	1.07	1.38	1.41	1.31	0.177	0.573	0.176	0.228
Others	2.59	2.30	2.98	2.65	2.63	0.221	0.273	0.043	0.925
F:B ³ ratio	0.41	0.48	0.43	0.60	0.48	0.010	<0.001	<0.001	0.026

CON, control group; RPA, with supplementary 15 g/day RP-Lys and 5 g/day RP-Met; C, with supplementary 1.2 kg/day concentrate; RPA+C, with supplementary 15 g/day RP-Lys and 5 g/day RP-Met and 1.2 kg/day concentrate. ¹ Sequences obtained from all 24 ruminal fluid samples (average of four treatments); ² SEM, standard error of mean; n = 6 for each group. ³ RPA = effect of rumen-protected amino acids; C = effect of concentrate; RPA × C = interaction between concentrate and rumen-protected amino acids; ³ F:B ratio = relative abundance of Firmicutes/relative abundance of Bacteroidetes.