Table 3.
The primer sequence of each gene according to Genbank or other research.
| Gene Name 1 | Primer Sequence | Genbank No. |
|---|---|---|
| ß-actin | F: 5′-CTGGCACCTAGCACAATGAA-3′ R: 5′-ACATCTGCTGGAAGGTGGAC-3′ |
X00182.1 |
| NFκB | F: 5′-CCAGGTTGCCATCGTGTTCC-3′ R: 5′-GCGTGCGTTTGCGCTTCT-3′ |
D13719.1 |
| IFN-γ | F: 5′-CTCCCGATGAACGACTTGAG-3′ R: 5′-CTGAGACTGGCTCCTTTTCC-3′ |
Y07922 |
| IL-1ß | F: 5′-GCTCTACATGTCGTGTGTGATGAG-3′ R: 5′-TGTCGATGTCCCGCATGA-3′ |
NM_204524 |
| IL-6 | F: 5′-AGGACGAGATGTGCAAGAAGTTC-3′ R: 5′-TTGGGCAGGTTGAGGTTGTT-3′ |
NM_204628 |
| IL-10 | F: 5′-TTCAGCTTGGATGTGTGAGC-3′ R: 5′-TGTCAGTTCTGCATGCTTCC-3′ |
XM_025143715.1 |
| Claudin-1 | F: 5′-GGAGGATGACCAGGTGAAGA-3′ R: 5′-TCTGGTGTTAACGGGTGTGA-3′ |
NM_001013611.2 |
| MUC-2 | F: 5′-GCTACAGGATCTGCCTTTGC-3′ R: 5′-AATGGGCCCTCTGAGTTTTT-3′ |
NM_001318434.1 |
| Occludin | F: 5′-GTCTGTGGGTTCCTCATCGT-3′ R: 5′-GTTCTTCACCCACTCCTCCA-3′ |
NM_205128.1 |
| ZO-1 | F: 5′-AGGTGAAGTGTTTCGGGTTG-3′ R: 5′-CCTCCTGCTGTCTTTGGAAG-3′ |
XM_015278975.1 |
1 NFκB: Nuclear factor kappa B p 65; IFN-γ: Interferon-γ; IL-1ß: Interleukin-1ß; IL-6: Interleukin-6; IL-10: Interleukin-10; MUC-2: Mucin2; ZO-1: Zonula occludens 1.