Figure 3.

C57BL/6J mice (n = 5 per group) were infected with 10⁵ spores of C. difficile strain M7404 (RT027). Six hours post-infection and then every 12 h thereafter, mice were administered either 10% DMSO (blue) or 2.5 mg (100 mg/kg in 10% DMSO) of compound 40 (red) by oral gavage. Mice were monitored daily for survival (a) and weight loss relative to day 0, which was the day of infection (b). Fecal spore load at 1-day post-infection was determined by plating (c). Data are presented as CFU/gram feces, with each point representing a single mouse. Mouse cages were scored on day 1 post-infection for appearance (d) and mice were individually assessed for fecal consistency (e) and physiological appearance (f). Data represent the mean ±S.E.M. and statistical significance was assessed using a log-rank (Mantel–Cox) test or one-way ANOVA with a post hoc Tukey’s multiple comparison test.