TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) | |
|---|---|---|
| Gene knockout | ||
| MXAN_3991_UF | CGGGAAGTCCAGCACCACGC | |
| MXAN_3991_UR | ACGGATCCAACGGGCTGGGCATCCTGGG | |
| MXAN_3991_DF | TTGGATCGCTCATCTCGCCTCCTCGCC | |
| MXAN_3991_DR | CGTGACGGAGCCGCTGGTGT | |
| MXAN_3990_UF | CTCGTGAATGGTGGGTGACGGTGC | |
| MXAN_3990_UR | AAGGATCCGGCTGGGCGGGGAGTGGTGGT | |
| MXAN_3990_DF | TTGGATCCTGCCTTCAGCGCCGAGGTGGA | |
| MXAN_3990_DR | GTGGGCTGCTCCTGCTGCTGACTT | |
| MXAN_1441_UF | ATGGATCCGCCGCCGCCACTGCCTTCA | |
| MXAN_1441_UR | GTATCCACACCCGTCACTTCC | |
| MXAN_1441_DF | GACCGCACGGGGCTCTTCAAT | |
| MXAN_1441_DR | ATGGATCCTAGACGGAGGACGCCAACAC | |
| Gene transcription | ||
| Fa | GGCTGCTCCTGCTGCTGACT | |
| Ra | CGGCAGGGCCGCGTCCAGTC | |
| Fb | TGGCCTTCGCCTCCACCTCG | |
| Rb | GTATTCCATGGGCCGCCTCG | |
| Gene overexpression | ||
| MXAN_3991_OF | CCGAATTCGACAAGAAGGTGGAAGTGAAGG | |
| MXAN_3991_OR | ATTCTAGAGAAGCGGGTGAGGTGCAGATA | |
| pilAp-up | TTCCCGGGCTGGCGAACTACTTCCTGTCC | |
| pilAp-down | CTTCTCCGCCAGCTTGCTCATGGGGGTCCTCAGAGAAGGTTG | |
| MXAN_1441_OF | CAACCTTCTCTGAGGACCCCCATGAGCAAGCTGGCGGAGAAG | |
| MXAN_1441_OR | AAGGTACCCGGTCAAGCTGGACGTGTT | |
| Protein expression | ||
| MXAN_1441_PF | AGGGTACCAAAACCACCGCCGAGGATGAC | |
| MXAN_1441_PR | AATCTAGACACTGCCTTCAGCTTCTCCGC | |
| MXAN_3982_PF | AAGGTACCAGAGCTGCCACGCGGTGGGAG | |
| MXAN_3982_PR | ATTCTAGAGCGGCAGACCAGCTCGGCGTA | |
| MXAN_3991_PF | TTGGTACCGCAGCGGAGCCCTTCTTCTT | |
| MXAN_3991_PR | TTTCTAGACCCCTGCCCCGCTCCCACTCG | |
| RT-PCR | ||
| RTPCR_3982_F | ATCATATGGACTACGCCGAGCTGGT | |
| RTPCR_3982_R | ACGGGGTGCGGCTCATCCTGGGCG | |
| RTPCR_3991_F | GTGGGCTGCTCCTGCTGCTGA | |
| RTPCR_3991_R | GGCGTGCCGTCCTCCGTGTCCT | |
| RTPCR_3990_F | TTCAGCGTGAAGGTGAGCCG | |
| RTPCR_3990_R | CGCTGGAAGAGGTGTTGGAGGA | |
| RTPCR_5350_F | GCACGAGCACGTCAGCCTCT | |
| RTPCR_5350_F | AGGCGCGTGATTTCATCGAG | |
| 3-kbp dsDNA | ||
| FM13 | ACTATTACCCCCTCTGGCAAAACT | |
| RM13 | GAAACCAATCAATAATCGGCTGTC | |