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Expression of PETase and MHETase in I. sakaiensis strains. The ΔpyrF, Δpetase, and Δmhetase strains were grown in SV-TPA·2Na and analyzed by Western blotting using polyclonal antibodies against PETase (A) and MHETase (B). Red arrowheads indicate the predicted molecular weight positions of PETase and MHETase proteins. M, molecular marker; P, parent strain (ΔpyrF); Dp, Δpetase strain; Dm, Δmhetase strain.
