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Growth of I. sakaiensis ΔpyrF and its mutant strains. The parent (ΔpyrF), Δpetase, and Δmhetase strains were cultivated in SV-Ura (A), SV-Ura-PET (B), SV-Ura-TPA·2Na (C), and SV-Ura-EG (D). Circle, triangle, and square symbols indicate the parent, Δpetase, and Δmhetase strains, respectively. The means ± standard deviations from three independent growth measurements are shown.
