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PET degradation by I. sakaiensis ΔpyrF and its mutant strains. The parent (ΔpyrF) (a), Δpetase (b), and Δmhetase (c) strains were cultured with PET film in SV-Ura-PET at 30°C for 10 days. (A) SEM image of the degraded PET film surface. Scale bars represent 1 μm. Visual images of the corresponding samples are shown in Fig. S5. (B) Weight loss of PET films after cultivation relative to before cultivation. The means ± standard deviations are calculated from three independent experiments.
