FIG 4.
Measurement of membrane potential and CCCP sensitivity. (A) Measurement of membrane potential (ΔΨ) for B. glumae strains. Fluorescence ratio in the graph represents the red (595 nm)/green (530) emission ratio of JC-1 dye. Twenty-five micromolar CCCP added to B. glumae 336gr-1 (vec) was used as a control for loss of ΔΨ. *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not significant. (B) CCCP sensitivity of B. glumae strains. Serially log10-diluted cells of B. glumae 336gr-1 and the ΔdbcA strain transformed with control vector (vec) and pSC301 (DbcA) were spotted and grown on MH2 agar growth medium containing 100 μg/ml Tmp, no rhamnose, and either 10, 20, or 25 μM CCCP at 37°C for 48 h.