Bicarbonate treatment results in loss of B. glumae virulence in rice. (A) Virulence phenotype on rice panicles for control, B. glumae 336gr-1 cells with no sodium bicarbonate, and B. glumae 336gr-1 cells with 5 mM sodium bicarbonate. For the control, 5 mM sodium bicarbonate was directly sprayed on rice panicles. The images were taken at 10 days postinoculation, and disease severity score was determined with a 0 to 9 scale. (B) The line graph shows the disease progress for rice panicles inoculated with control (circles), B. glumae 336gr-1 cells with no sodium bicarbonate (squares), and B. glumae 336gr-1 cells with 5 mM sodium bicarbonate (triangles) at 3, 7, and 10 days postinoculation. (C) Detailed view of seed discoloration observed by indicated treatments at 10 days postinoculation.