TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strain | ||
| Escherichia coli | ||
| XL1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac [F′ proAB lacIqZΔM15] Tn10 (Tetr) | Stratagene |
| RHO3 | SM10(λpir) Δasd::FRT ΔaphA::FRT, Kans | 64 |
| Burkholderia glumae | ||
| 336gr-1 | Wild-type Burkholderia glumae | 65 |
| ΔdbcA strain | 336gr-1 ΔdbcA::Tmpr | This study |
| ΔdbcA::FRT strain | 336gr-1 ΔdbcA::Tmps | This study |
| Plasmid | ||
| pUC18T-mini-Tn7T-Tp | Mini-Tn7 T-based vector containing a trimethoprim resistance cassette; Tmpr (GenBank accession no. DQ493875) | 69 |
| pEX18Gm | oriT+sacB+, gene replacement vector with multiple cloning site from pUC18, Gmr | 63 |
| pEX3525 | Assembled upstream and downstream fragment of dbcA gene interrupted by Tmpr fragment cloned into pEX18Gm | This study |
| pFlpTet | Rham-inducible flp, TS ori | 66 |
| pSCrhaB2 | Expression vector; oripBBR1rhaR, rhaS, PrhaBTmprmob+ | 68 |
| pSC301 | pSCrhaB2 expressing B. glumae dbcA | This study |
| pBBR1MCS-2 | Expression vector; RK2mob lacZα E. coli lac promoter, Kanr | 70 |
| pMCS501 | pBBR1MCS-2 expressing B. glumae dbcA with dbcA promoter | This study |
| pRP101 | pBBR1MCS-2 expressing B. thailandensis dbcA | 5 |
| pRP102 | pBBR1MCS-2 expressing E. coli yqjA | 5 |
| pRP103 | pBBR1MCS-2 expressing E. coli yghB | 5 |