TABLE 2.
Oligonucleotide primers used in this study
| Primer name | Sequence (5′→3′)a |
|---|---|
| K1F | ATTAGGTACCAACACGTACTGGTGGATCGAG |
| K1R | TCCCCAATTCGAGCTCATCAGCGTATCCAAGACGTTTCCTT |
| K2F | ACGTCTTGGATACGCTGATGAGCTCGAATTGGGGATCTTGAAGTA |
| K2R | TCCCACCAGCACGATCACCCACTAGTGAGCTCATGCATGAT |
| K3F | CATGAGCTCACTAGTGGGTGATCGTGCTGGTGGGAGTC |
| K3R | ATATAAGCTTCTGTTCCACAGCACCTCGTTT |
| Fw_pEX18Cont. | AGGCAAATTCTGTTTTATCAGACCGC |
| Rev_pEX18Cont. | GAGCGGATAACAATTTCACACAGGAA |
| Seq 1 | ATACTTCTTCGTCAACGGCCG |
| Seq 2 | AGGCGTGTTGAAGTACAGCTC |
| Seq 3 | GTGGAATCGGTGGAATCGGTA |
| Seq 4 | GCATAGCCTTCAGGAGTGAGT |
| Fw_Seq 5A | CGATCATGCATGAGCTCACTAGT |
| Rev_Seq 5A | TCAAGGTCGGCGACTCGATCT |
| Seq 5 | TCGCAATAGACCTGCCAGTAG |
| Seq 6 | AGGATCAGCACGATGGTCAGC |
| KO-FW | AACCGAAGGAAACGTCTTGGATACG |
| KO-REV | AGCTACTGGCAGGTCTATTGCGA |
| FW-pSCdbcA | CGCCCATATGATGGATACGCTGCTTCACTTCGTCAATC |
| REV-pSCdbcA | CGCGAAGCTTTCAGCCGCGCCCGCGCG |
| Fr-Seq-pSCdbcA | CATCATCACGTTCATCTTTCCCTG |
| Rv-Seq-pSCdbcA | GCAAATTCTGTTTTATCAGACCGC |
| pMCSdbcA-FW | ATTAGGTACCATTCGGACATGCGGGAATTATAACGACG |
| pMCSdbcA-REV | CGCGAAGCTTTCAGCCGCGCCCGCGCG |
| SeqM13-FW | TGTAAAACGACGGCCAGTGAG |
| SeqM13-REV | TCACACAGGAAACAGCTATGA |
a
Underlined text represents homology sequences. Bolded italic text represents restriction enzyme sites.